Abstract: Thirteen allozymes,α-glycerophosphate dehydrogenase (α-GPDH), acid phosphatase (ACPH), alkaline phosphatase (ALP), aldehyde oxidase (AO), esterase (EST), glutamate oxaloacetate transaminase (GOT), hexokinase (HEX), leucine aminopeptidase (LAP), lactate dehydrogenase (LDH), malate dehydrogenase (MDH), malate enzyme (ME), phosphoglucomutase (PGM), xanthine dehydrogenase (XDH), were analysed by polyacrylamide gradient gel electrophoresis with double stain method. The genetic variations of 9 allozymes within and among 5 geographic populations were investigated. Among the 13 loci examined, 6 loci were polymorphic and 7 loci were monomorphic. The percentage of polymorphic loci was 46.15%. As calculated with the results of AO, GOT, LAP, LDH, ME and XDH, the average heterozygosity of Helicoverpa armigera was 0.1160.Among Nanjing, Chengdu, Wuxue , Hengyang and Hami populations, their mean genetic distance was 0.0008～0.0293 and mean genetic similarity was 0.9707～0.992. It means that there exits high genetic diversity in each population, but the genetic differentiation among these populations is very limited. There was no barrier of gene flow among these investigated populations. The migration could hamper more genetic differentiation among different geographic populations.

Key words: allozyme, genetic variation, Helicoverpa armigera (Hübner)