Abstract: Sequence analysis of the p35 gene of Hyphantria cunea nucleopolyhedrovirus (HycuNPV) showed that the HycuNPV p35 gene was 900 bp and encoded 299 amino acids. Compared with BomoNPV T3, AucaNPV, SpliNPV, LeseNPV and HearNPV, nucleotide and amino acid identity were 99.9%, 95.7%, 93.6%, 80.2%, 87.2% and 99.7%, 90.3%, 77%, 64.9%, 732% respectively, and showed evolutionary conservation. H¹²² of BomoNPV T3 p35 was replaced by R in HycuNPV p35. It is possible that the function and anti-apoptotic action of HycuNPV p35 is similar to that of BomoNPV T3 p35.

Key words: Hyphantria cunea nucleopolyhedrovirus, p35 gene, nucleotide sequence