Abstract: The protein concentrations of cell supernatants infected with recombinant AcNPV (Autographa californica nuclear polyhedrosis virus )containing wild type or mutant juvenile hormone esterase were between 153.32-188.0 μg/mL. The enzyme activity recovery rate was about 33%. The stepwise purification of the enzyme had similar results as the gradient method, but was more convenient for handling large volumes of cell supernatants. The cuticle blackening te st showed that the recombinant juvenile hormone esterase from Heliothis virescens had biological effects on tobacco horn moth larvae（Manduca sexta）.

Key words: recombinant juvenile hormone esterase, biological assay, Manduca sexta, ion exchange chromatography