›› 2003, Vol. 46 ›› Issue (2): 254-258.

• RESEARCH PAPERS • Previous Articles    

Cloning and sequencing of cDNA encoding prepromelittin in Apis cerana cerana and Apis mellifera

SHI Wan-Jun, ZHANG Su-Fang, ZHANG Chuan-Xi, CHENG Jia-An*   

  • Online:2003-04-20 Published:2003-04-20

Abstract: Two cDNA fragments encoding prepromelittin were amplified by RT-PCR from the total RNA from the venom glands of the worker honeybees, Apis cerana cerana and A. mellifera. The PCR products were ligated into pGEM-Teasy vector. The results of sequencing showed that the ORFs coding for prepromelittin were all 213 bp in length. The amino acid sequence of A. cerana cerana prepromelittin shared 97% homologies with that of A. mellifera prepromelittin and A. cerana indica prepromelittin. The nucleotide sequence reported here was submitted to GenBank with accession no. AF487907.

Key words: Apis cerana cerana, Apis mellifera, prepromelittin, clone, sequence analysis