Abstract: To explore a new method to control arthropod-borne diseases, prM gene from dengue virus was inserted into a transposon piggyBac based plasmid and an Insect transgenic vector pB［PUBnls-EGFP-prM］ was constructed. The vector was used to transfect Aedes albopictus C6/36 cell with a helper plasmid. PCR and Southern blot tests showed that EGFP-prM gene had integrated into C6/36 cell genome. The results indicate that transposon piggyBac element is functional in Ae. albopictus and should be useful in further constructing dengue-refractory transgenic Ae. Albopictus.

Key words: Aedes albopictus C6/36 cell, prM gene, piggy Bac element, transposon