运用RAPD技术对棺头蟋属昆虫亲缘关系的研究

Abstract

Abstract: The technique of random amplified polymorphic DNA (RAPD) was used to study the relationships of 9 species in the genus Loxoblemmus. A total of 27 samples were used in this study: 3 samples for each species. Among 54 arbitrary primers (10 bp) under predetermined optimal reaction conditions, 9 primers were informative and yielded a total of 193 clear and reproducible bands. Dendrograms were constructed based on genetic distance by the UPGMA method. Individuals of the same species clustered together. The relationships indicated by cluster analysis was mostly the same as those based on comparative morphology.

Key words: Orthoptera, Grylloidea; Loxoblemmus, relationship, RAPD

LI Kai, ZHENG Zhe-Min, CHEN Li-Qiao. Analysis of relationships among species in the genus Loxoblemmus (Orthoptera : Grylloidea, Gryllidae) using random amplified polymorphic DNA（RAPD）[J]., 2003, 46(6): 761-765.

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http://www.insect.org.cn/EN/Y2003/V46/I6/761

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Analysis of relationships among species in the genus Loxoblemmus (Orthoptera : Grylloidea, Gryllidae) using random amplified polymorphic DNA（RAPD）

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