›› 2004, Vol. 47 ›› Issue (4): 424-428.

• RESEARCH PAPERS • Previous Articles     Next Articles

Transmission of dengue 2 virus by diapausing eggs of Aedes albopictus

GUO Xiao-Xia1, ZHAO Tong-Yan1*, DONG Yan-De1, JIANG Shu-Nan2, LU Bao-Lin1   

  • Online:2004-08-20 Published:2004-08-20
  • Contact: ZHAO Tong-Yan

Abstract: Virus isolation in C6/36 cells was used for detection of the infection rates of F1 progeny from diapausing eggs of infected Aedes albopictus. Eggs from three gonotrophic cycles (GC) were collected, and vertical transmission was not observed in the first gonotrophic cycle, while there was no significant difference observed between the infection rates of the GC2 progeny and that of the GC3 progeny (P>0.05). The total positive rate of pools was 9.1%, and the minimum infection rate was 1∶330. The results of indirect fluorescent antibody test showed that F1 adult mosquitoes obtained from diapausing eggs can transmit dengue 2 virus to sensitive mice horizontally through biting. The horizontal transmission rate was 6.1%. These results indicate that dengue 2 can be transmitted vertically even the infected eggs are induced into diapausing, and F1 adult mosquitoes raised from diapausing eggs can transmit dengue 2 virus to sensitive mice horizontally via biting.

Key words: Aedes albopictus, dengue 2 virus, transovarial transmission, horizontal transmission