›› 2004, Vol. 47 ›› Issue (4): 454-460.

• RESEARCH PAPERS • Previous Articles     Next Articles

Dynamics of Cry1Ab protein from transgenic Bt rice in herbivores and their predators

JIANG Yong-Hou1,2,3, FU Qiang3, CHENG Jia-An1*, ZHU Zeng-Rong1, JIANG Ming-Xing1, YE Gong-Yin1, ZHANG Zhi-Tao3   

  • Online:2004-08-20 Published:2004-08-20
  • Contact: CHENG Jia-An

Abstract: Levels of Cry1Ab protein in several herbivores and their predators through food chain from transgenic Bt rice (KMD1) were studied in the laboratory. The results showed that the levels of Cry1Ab in stripped stem borer (SSB) Chilo suppressalis continuously feeding on KMD1 or on Xushui 11 control rice after feeding on KMD1 for 36 h declined with time at the booting stage as well as at the maturing stage. Levels of Cry1Ab in Mycalesis gotama continuously feeding on KMD1 or on Xushui 11 control rice after feeding on KMD1 for two days declined with time, but more dramatically than those of SSB. High levels of Cry1Ab were detected in faeces of both C. suppressalis and M. gotama, but no Cry1Ab detected in their controls. 3.5 ng/g Cry1Ab was detected in the haemolymph of C. suppressalis feeding on KMD1 Different levels of Cry1Ab were all detected in brown planthopper (BPH) Nilaparvata lugens, rice aphid (RA) Sitobion avenae reared on KMD1, and wolf spider (WS) Pirata subpiraticus fed with preys that had ingested KMD1. The concentration of Cry1Ab protein in P. subpiraticus fed with M. gotama larvae reared on KMD1 was about 60 times higher than that in P. subpiraticus fed with C. suppressalis reared on KMD1 for 36 h, and no Cry1Ab protein was detected in their controls. These results indicated Cry1Ab protein could be transferred along food chain.

Key words: Transgenic Bt rice, Cry1Ab protein, herbivore, predator, food chain