›› 2004, Vol. 47 ›› Issue (4): 522-525.

• RESEARCH PAPERS • Previous Articles     Next Articles

Cloning and sequence analysis of an ubiquitin gene of Blattella germanica

YU Hang, JIN Feng-Liang, XU Xiao-Xia, YANG Xing, GU De-Xiang, ZHANG Wen-Qing*   

  • Online:2004-08-20 Published:2004-08-20
  • Contact: ZHANG Wen-Qing

Abstract: The coding sequence of Blattella germanica ubiquitin gene was cloned and determined (GenBank Accession No. AY501003). The length of this opening reading-frame (ORF) is 228 bp, encoding a protein with molecular weight of 8.47 kD and isoelectric point of 5.73.Multiple sequence alignment indicated that B. germanica ubiquitin was very similar to the homologous proteins of other eukaryotic species and shared more than 94% identity with other eukaryotic ubiquitins at amino acid level.

Key words: Blattella germanica, ubiquitin gene, cloning, sequence analysis