›› 2005, Vol. 48 ›› Issue (6): 922-927.

• RESEARCH PAPERS • Previous Articles     Next Articles

Vertical transmission of Spodoptera exigua nuclear polyhedrosis virus in the beet armyworm, Spodotera exigua

JIANG Jie-Xian, ZENG Ai-Ping, JI Xiang-Yun, JIANG Zhu-Ru   

  1. Shanghai Academy of Agricultural Sciences
  • Online:2005-12-29 Published:2005-12-20

Abstract:

The experiments were conducted to determine the transmission mode and rate of Spodoptera exigua nuclear polyhedrosis virus (SeNPV) from the infected parents to their progeny through feeding the 4th and 5th instar larvae with the concentrations of 2.8×107 and 2.8×106 PIBs·mL-1 in the laboratory. The results showed that  SeNPV could be transmitted to the offspring by three modes, i.e. transovarian, transovum, and adult mating. The female-mediated vertical transmission test indicated that the adults developed from virus-infected larvae transmitted the virus to their progeny, resulting in 20%-48% mortality of the next generation (F1) and decrease of the fecundity of F1 progeny adults compared with untreated controls. Moreover, the experiment with surface of the egg sterilized by 5% formalin solution showed that SeNPV could be transmitted to larvae of F1 generation by transovarian transmission, which caused 18.8% mortality of F1 generation. The crossing experiments between virus-infected and non-infected females and  virus-infected and non-infected males indicated that virus could also be transmitted to F1 generation by sperm of male adult, which caused 20%-40% mortality of offspring larvae. Furthermore, through the experiment of adult oral infection, it was found that SeNPV could also be transmitted to offspring at a prevalence rate of 28.6%.

 

Key words: Spodoptera exigua, nuclear polyhedrosis virus, vertical transmission, transovarian transmission, transovum transmission, adult mating transmission