The classificatory schema in some groups of Megachilidae reorganized by means of shape differences in terms of the inter- and intra-specific and inter- and intra-generic and subgeneric deformations. The relative warps, UPGMA trees and PCA graphs obtained from 30 landmarks were shown. Both Anthidium cingulatum Latreille and A. florentinum (Fabricius) should better classify in the same subgenus Anthidium s. str. In the other case under the results of shape variation our results supported the hypothesis that Paraanthidium as a subgenus of Anthidium Fabricius. On the other hand Icteranthidium Michener and Paraanthidiellum Michener showed enough dissimilarity to be kept as separate genera. Our analysis also supported the monophyletic origin of Megachilini which was clustered as a separate group by all methods used.