›› 2006, Vol. 49 ›› Issue (3): 528-532.

• RESEARCH PAPERS • Previous Articles     Next Articles

The comparison of yeast-like symbionts in the brown planthopper, Nilaparvata lugens Stal with different virulence based on partial 18S rDNA sequence

ZHANG Jue-Feng, LU Zhong-Xian, CHEN Fa-Jun, CHEN Jian-Ming, ZHENG Xu-Song, XU Hong-Xing, CHEN Lie-Zhong, YU Xiao-Ping   

  1. Zhejiang Academy of Agricultural Sciences
  • Online:2006-07-10 Published:2006-06-20

Abstract:

The yeast-like symbionts (YLS) were isolated and purified from the fat body of the brown planthopper, Nilaparvata lugens Stal (BPH) with different virulence reared on various rice resistant varieties. The 18S rDNA sequences of YLS from BPH with different virulence were analyzed and compared with the sequences of other fungi explored from GenBank, and a comprehensive phylogenetic tree was constructed. The phylogenetic analyses suggested that the YLS of BPH with different virulence belonged to the subphylum Ascornycotina, the class Pyrenomycetes, and had the closest genetic relationship with Hypomyces chrysospermus.
 

Key words: Nilaparvata lugens, yeast-like symbionts, Ascomycotina, Pyrenomycetes, virulence, 18S rDNA, molecular phylogenetic tree