›› 2009, Vol. 52 ›› Issue (2): 223-227.
• RESEARCH PAPERS • Previous Articles Next Articles
Online:
Published:
Abstract: In this study, the ISSR-PCR reaction system was established, optimized and then used to analyze the genetic coefficient and the subfamily composition of Bombus hypocrita Pérez.The optimized reaction system was established as follows: 1×PCR buffer, 0.3 mmol/L dNTP, 1.25 μmol/L primer, 3 mmol/L Mg2+, 2.5U Taq polymerase and 8.18-23.76 μg/mL DNA. Our results indicated that ISSR markers obtained with this optimized system could be effectively used to analyze the genetic coefficient and subfamily composition.
Key words: Bumble bee, Bombus hypocrita, ISSRPCR, clustering analysis, genetic coefficient, copulation
0 / / Recommend
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
URL: http://www.insect.org.cn/EN/
http://www.insect.org.cn/EN/Y2009/V52/I2/223
Cited