Abstract: The inhibition of glutathione S-transferase (GSTs) activity in 4 tissues (midgut, fat body, head and integument) of Clostera anachoreta (Fabricius) by 21 insecticides and 3 allelochemicals was conducted using the biochemistry methods in order to compare the difference of GSTs in various tissues. The results showed that the inhibition of GST activity of different tissues by 21 insecticides and 3 allelochemicals was different. For GST activity in the head, chlorpyrifos, fipronil, quercetin and tannic acid were the best inhibitors tested; while in midguts, quercetin and tannic acid were the most potent inhibitors tested; for GST activity in the fat body, the inhibitory effect of tannic acid was the highest, and the inhibitory effects of phoxim, lambda-cyhalothrin, deltamethrin and endosulfan were the highest for GST activity in the integument. The results suggest that the difference in the sensitivity of GSTs to 21 insecticides and 3 allelochemicals in different tissues of C. anachoreta might be due to the quantitative difference in isoezyme composition.

Key words: Clostera anachoreta, glutathione S-transferases, plant allelochemicals, insecticides, inhibition