甜菜夜蛾几丁质合成酶基因A和B启动子的克隆

Abstract

Abstract: Chitin, synthesized by chitin synthase (CHS), is not only the essential component for epidermis and peritrophic membrane of the midgut in insects, but also a very pivotal target for insect pest control. Based on the cDNA sequences and genomic sequences of the two CHS genes in Spodoptera exigua, the 5′flanking sequences were cloned using specific primers. Promoter sequences of SeCHSA and SeCHSB were obtained after initiation sites of transcriptional regulation were verified by means of 5′RACE. These results are important for the research of chitin synthesis and transcriptional regulation in insects.

Key words: Spodoptera exigua, chitin, chitin synthase, promoter, cloning

TANG Bin,WANG S G, ZHANG W Q. Cloning of chitin synthase gene A and B promoters from Spodoptera exigua (Lepidoptera: Noctuidae)[J]., 2009, 52(7): 736-742.

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Cloning of chitin synthase gene A and B promoters from Spodoptera exigua (Lepidoptera: Noctuidae)

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