Abstract:

Cyrtotrachelus buqueti Guerin-Meneville, a main bamboo pest, specializes in boring and damaging the top of tufting bamboo shoot. For analyzing the reason, the antennal ultrastructure of C. buqueti and the volatiles of different bamboo shoots and the antennal olfactory responses of female to the volatiles were studied with the scanning electron microscoopy and the gas chromatographic-mass spectrometry (GC-MS) and the electroantennogram (EAG), respectively. The results showed that the sensilla basiconica, with the ability of apperceiving the volatiles of bamboo shoot, was the most sensilla on the antenna of C. buqueti, and 28 bamboo shoot volatile compounds from bamboo shoot were discovered by GC-MS, in which the relative content of aldehydes was the highest, and those of alcohols and acids come second. The EAG analysis deduced that the relative EAG values of benzaldehyde with the highest relative EAG values and other 12 volatiles were significantly higher than the control. These results suggest that the high content or the special volatiles of the top of tufting bamboo shoot might be the important odor signals which allure C. buqueti to tend towards the top of bamboo shoot, and that C. buqueti might identify the bamboo shoot by the chemistry finger map with primary benzaldehyde and special proportion of components. This study provides a theoretical basis for developing attractant of C. buqueti.

Key words: Cyrtotrachelus buqueti, antenna, ultrastructure, antennal sensilla, bamboo shoot volatile, electroantennogram