›› 2010, Vol. 53 ›› Issue (11): 1227-1232.
• RESEARCH PAPERS • Previous Articles Next Articles
JIANG Lei, LI Guo-Xun, LI Chang-You, Robert R. GRANADOS, Gary W. BLISSARD
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Abstract:
The development of new cell lines from unusual and non-pest species may provide important source for basic research and biotechnology application. In this study, three new insect cell lines designated as QB-Ms1-8, QB-Ms2-2, and QB-Ms2-7, respectively, were established from eggs of Manduca sexta. These cells were subcultured approximately 50 passages in TNMFH medium at 28℃. The majority of cells of the lines generally are spindle-shaped. The population doubling time of QB-Ms1-8, QB-Ms2-2 and QB-Ms2-7 cells was 51, 31 and 49 h, respectively. Although these lines were insusceptible to Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) with the infection rate of 33%-40% at 96 h post infection, QB-Ms2-2 cell line showed higher level of secreted SEAP production in comparison with BTI-Tn5B1-4 cell line. In this study, a SEAP high expression type cell line, QB-M2-2 was isolated from the three new cell lines established from M. sexta, which may provide a source for searching novel clones with higher production of recombinant proteins.
Key words: Manduca sexta, insect cell line, recombinant protein, growth curve, Autographa californica multiple nuclear polyhedrosis virus (AcMNPV)
JIANG Lei, LI Guo-Xun, LI Chang-You, Robert R. GRANADOS, Gary W. BLISSARD. Growth characteristics and expression of recombinant proteins in three new cell lines from Manduca sexta (Lepidoptera: Sphingidae)[J]., 2010, 53(11): 1227-1232.
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http://www.insect.org.cn/EN/Y2010/V53/I11/1227
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