Abstract: To determine the localization of cross-reactivity allergens in the body of Tarsonemus granarius, paraffin sections of T. granarius stained by hatmatoxylin-eosin (HE) were observed by optical microscope. Serum specific IgE antibodies from patients who were sensitive to the house dust mite served as probe, and the cross-reactivity allergens in the body of T. granarius were localized by the method of immunohistochemistry. The digestive system was found to occupy large parts of body cavity of T. granarius by HE staining, which consists of oropharynx, midgut, hindgut, anus, salivary gland and so on. The cross-reactivity allergens of T. granarius mostly occurred in the midgut tissue, gut contents, cuticle and reproductive system in the immunostained sections. The body of T. granarius has allergens which can trigger IgE antibodies of the human body and the distribution of allergens in its body is consistent with that in the house dust mite, Dermatophagoides pteronyssinus.

Key words: Tarsonemus granarius, Tarsonemidae, allergen, antigenic localization, immunohistochemistry, hatmatoxylin-eosin stain, cross-reactivity