›› 2012, Vol. 55 ›› Issue (11): 1306-1314.doi:

• RESEARCH PAPERS • Previous Articles     Next Articles

Sequencing and analysis of the complete mitochondrial genome of Hylobitelus xiaoi (Coleptera: Curculionidae)

LI Guo-Hong, SHANG Na, WEI Jian-Rong   

  • Received:2012-09-24 Revised:2012-11-19 Online:2012-11-20 Published:2012-11-20
  • Contact: WEI Jian-Rong E-mail:weijr@hbu.edu.cn
  • About author:lmz9827@yahoo.com.cn

Abstract: The Curculionoidea is one of the richest groups in Coleptera, but the research about its complete mitochondrial genome has not been reported. In this study, the complete mitochondrial genome (mitogenome) of Hylobitelus xiaoi Zhang was determined using long PCR (L-PCR) and primer walking technology. The entire mitochondrial DNA (mtDNA) molecule is 16 123 bp in length and its overall nucleotide composition is heavily biased towards As and Ts, which account for 75.66% of the whole genome. The H. xiaoi genes are arranged in the same order and orientation as the other hexapod species, except for the occurrence of one 69 bp trnV-like region with anticodon GAC that overlaps 65 bp with the reversely encoded trnD and the presence of 232 bp long intergenic spacer between trnSUCN and nad1. All protein-coding genes (PCGs) start with a typical ATN codon. Nine of 13 PCGs have a complete termination codon TAA, while the remaining nad1 and cox2 are terminated with TAG, nad4 and nad5 with the incomplete T. All tRNAs show the typical clover-leaf structure, except for trnSAGN. It habors anticodon TCT instead of GCT, a lengthened 9 bp anticodon stem with a bulged nuleotide in the middle, an unusual 6 bp TΨC-stem, and a mini 1 bp DHU-stem and no connector nucleotides. The control region (CR) contains one 14 bp Poly-T sequence (ten Poly-T sequences more than 5 bp and two microsatellite-like repeating sequences (TA)6 and (TA)9. The results of this study will provide some important molecular data for discovering phylogenetic relationships among the superfamilies of Coleptera.

Key words: Coleoptera, Curculionoidea, Hylobitelus xiaoi, mitochondrial genome, long PCR (LPCR), primer walking