›› 2012, Vol. 55 ›› Issue (3): 357-360.doi:

• SHORT COMMUNICATIONS • Previous Articles     Next Articles

Assay and comparison of chemical constituents of crude methanol extracts from different developmental stages of darkling beetle Zophpbas morio (Coleoptera: Tenebrionidae)

CHEN Wen-Ting, LIU Hai-Li, YAO Li-Gong, YE Zhu-Liang, GUO Yue-Wei   

  1. State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
  • Received:2011-08-18 Revised:2012-02-28 Online:2012-03-20 Published:2012-03-20
  • Contact: GUO Yue-Wei E-mail:ywguo@mail.shcnc.ac.cn
  • About author:windychen1110@gmail.com

Abstract: To study the chemical changes of darkling beetle Zophobas morio during metamorphosis, the crude methanol extracts of Z. morio in three developmental stages (larva, pupa and adult) were assayed by modern chromatographic techniques, 1H NMR and GC-MS and compared. The results showed that pupae contained characteristic chemical constituents. The methanol extract of the pupae was systematically studied and ten fatty acids were identified. 10-oxohexadecanoic acid and 10-oxooctadecanoic acid were found from pupae of Z. morio, which were separated from insects for the first time. (8E, 11E)-8, 11-octadecenoic acid, (9Z, 12Z)-9, 12-octadecenoic acid, (9Z, 12Z, 15Z)-9, 12, 15-octadecenoic acid, 8-(3-octyl-2-oxiranyl)octanoic acid, and 8-(2-octylcyclopropyl)octanoic acid were also found from Z. morio for the first time. These results offer the new theoretical basis for the utilization of darkling beetle at different developmental stages.

Key words: Zophobas morio, pupa, crude methanol extract, chemical constituent, 10-oxohexadecanoic acid, 10-oxooctadecanoic acid

CLC Number: 

  • Q968