›› 2012, Vol. 55 ›› Issue (4): 482-487.doi:

• SHORT COMMUNICATIONS • Previous Articles     Next Articles

Phylogenetic position of yeast-like symbiotes from three rice planthoppers (Homoptera: Delphacidae) based on 18S rDNA and ITS-5.8S rDNA sequences

ZHOU Yan-Yan, DONG Sheng-Zhang, BAI Xu, YU Xiao-Ping   

  • Received:2011-12-05 Revised:2012-03-28 Online:2012-04-20 Published:2012-04-20
  • Contact: YU Xiao-Ping E-mail:yxp@cjlu.edu.cn
  • About author: zhouyanyan861205@163.com

Abstract: In order to study the species status of yeast-like symbiotes (YLS) from three rice planthoppers (Laodelphax striatellus, Nilaparvata lugens and Sogatella furcifera) and the evolutionary relationship of YLS with insect hosts, the complete sequences of 18S rDNA and ITS-5.8S rDNA of YLS from the three planthoppers were determined. Sequence identity comparison of YLS from rice planthoppers was conducted based on 18S rDNA and ITS-5.8S rDNA sequences. The results showed that YLS of N. lugens has a higher identity to that of S. furcifera (98.91%) than to that of L. striatellus (95.74%) according to 18S rDNA sequences, but YLS of L. striatellus has a higher identity to that of S. furcifera (99.57%) than that to N. lugens (91.91%) according to the ITS-5.8S rDNA sequences. Phylogenetic analysis based on both 18S rDNA and ITS-5.8S rDNA showed that YLS from three rice planthoppers has no obvious identity to other known fungi. The results suggest that insect YLS have a close evolutionary relationship with hosts and form a taxonomic group different from other fungi.

Key words: Rice planthopper, Laodelphax striatellus, Nilaparvata lugens, Sogatella furcifera, yeast-like symbiotes (YLS), 18S rDNA, ITS-5.8S rDNA, phylogenetic analysis