家蝇幼虫血淋巴抗真菌肽MAF-1的分子特性分析及其抗真菌作用

›› 2012, Vol. 55 ›› Issue (9): 1046-1053.doi:

• RESEARCH PAPERS • Previous Articles Next Articles

Characterization of the antifungal peptide MAF-1 from the hemolymph of Musca domestica larvae and its antifungal activity

TU Tao-Tian, JI Heng-Qing, GUO Guo, FU Ping, WU Jian-Wei

Received:2012-07-28 Revised:2012-09-09 Online:2012-09-20 Published:2012-09-20
Contact: WU Jian-Wei E-mail:wjw@gmc.edu.cn
About author:tuqian836@sohu.com

Abstract

Abstract: To study the antifungal mechanism of Musca domestica antifungal peptide, MAF-1 was successfully prepared by solid phase extraction (SPE) and RPHPLC from the hemolymph of M. domestica larvae. MAF-1 exists mainly as α-helices and β-sheets in aqueous solution, as revealed by analysis of circular dichroism spectra. The results detected by scanning electron microscopy showed that the membranes of some Candida albicans fungi were depressed, shrinked and ruptured at 1 h after treatment with MAF-1. The results of single-cell gel electrophoresis showed that the “comet”-like cells were observed in the experimental group; however, typical round fungi were found in the normal control group. SDS-PAGE analysis showed that some protein bands were significantly weaker, the number of bands decreased and some bands even disappeared when C. albicans was treated with MAF-1. The results suggest that MAF-1 may have a unique antifungal mechanism.

Key words: Musca domestica, MAF-1, circular dichroism spectra, single-cell gel electrophoresis, antifungal mechanism

TU Tao-Tian, JI Heng-Qing, GUO Guo, FU Ping, WU Jian-Wei. Characterization of the antifungal peptide MAF-1 from the hemolymph of Musca domestica larvae and its antifungal activity[J]., 2012, 55(9): 1046-1053.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.insect.org.cn/EN/

http://www.insect.org.cn/EN/Y2012/V55/I9/1046

[1]	GU Ji-Hai, LI Ya-Jing, LI Wen-Qian, TANG Ting, LIU Feng-Song. Expression profiles of Mdsirt1 in Musca domestica (Diptera: Muscidae) under stresses of bacteria, high temperature and heavy metal [J]. , 2017, 60(12): 1394-1402.
[2]	ZHANG Jun-Tian, XIONG Guo-Hong, HE Jian-Guo, XU Ying, WEI Li-Xiu, PENG Ying-Hui. Fumigation activities of three essential oils against adult Musca domestica (Diptera: Muscidae) [J]. , 2016, 59(6): 641-646.
[3]	LU Yong-Peng, GU Ji-Hai, GAO Yi-Fu, TANG Ting, LIU Feng-Song, HUANG Da-Wei . Functional analysis of peptidoglycan recognition protein PGRP-SC in intestinal immunity of Musca domestica [J]. , 2016, 59(3): 269-277.
[4]	XING Jian-Xiao, LIU Yan-Juan, LU Yong-Peng, TANG Ting, LIU Feng-Song. Cloning and functional analysis of C-type lectin from Musca domestica (Diptera: Muscidae) [J]. , 2016, 59(1): 16-24.
[5]	LI Lin, XIONG Xin, MA Shu-Jie, MA Zhi-Qing , ZHANG Xing. Comparison of the insecticidal activities of terpinen-4-ol optical isomers and racemate against the housefly, Musca domestica [J]. , 2015, 58(7): 761-766.
[6]	YANG Xue, TANG Ting, WANG Yi-Li, LIU Xin, CAO Xin-Ru, NI Zhi-Hua, LIU Feng-Song. Gene cloning, expression profiling and anti-microbial assay of Muscin, a new antimicrobial peptide in the housefly (Musca domestica) [J]. , 2015, 58(6): 617-624.
[7]	LI Ke, LI Xiang-Hui, XU Xi-Lin, YUAN Zhe-Ming. Nonlinear quantitative structureactivity relationship of the aromatic carboxylic acid repellents [J]. , 2014, 57(9): 1018-1024.
[8]	ZHU Cheng-Jie, HE Zhang, CHEN Wei, ZHANG Shi-Lin, HU Hao-Yuan. Comparison of life table parameters of the laboratory population of Spalangia endius (Hymenoptera: Pteromalidae) on frozenkilled and fresh housefly pupae [J]. , 2014, 57(10): 1219-1226.
[9]	QIAN Cen, FANG Qi, WANG Lei, YE Gong-Yin. Functional studies of pacifastin proteinase inhibitor genes, nvpp-1 and nvpp-2, in Nasonia vitripennis (Hymenoptera: Pteromalidae) [J]. , 2013, 56(8): 841-853.
[10]	LU Dan, ZHENG Li, WANG Xin-Xin, WANG Fan, TANG Ting, LIU Feng-Song. Cloning, induced expression and promoter activity of defensin-1 gene from the housefly (Musca domestica) [J]. , 2013, 56(8): 854-863.
[11]	TANG Ting, GAO Yi-Fu, LIU Yan-Juan, GE Xu-Dong, LI Xiang, LIU Feng-Song. Identification of the gene MdSOD3 and its role in resisting heavy metal stress in housefly (Musca domestica) [J]. , 2012, 55(3): 267-275.
[12]	HUANG Ji-Guang, Zhao-Huan-Huan, Miao-Hui, Xu-Han-Hong. Insecticidal activities and active ingredients of Cacalia tangutica against Musca domestica and Aedes albopictus [J]. , 2012, 55(12): 1355-1361.
[13]	LIU Feng-Song, SUN Ling-Ling, TANG Ting, WANG Li-Na. Cloning, sequence analysis and induced expression of attacin-2 gene in housefly (Musca domestica) [J]. , 2011, 54(1): 27-33.
[14]	LI Dian-Xiang, KANG Cui-Jie, ZHANG Wei, WANG Jin-Xing, ZHAO Xiao-Fan. Construction of a suppression subtractive hybridization cDNA library to identify differentially expressed genes from Musca domestica (Diptera: Muscidae) larvae [J]. , 2010, 53(6): 601-610.
[15]	LIU Feng-Song, DONG Xiao-Yin, ZHANG Rui-Ying, TANG Ting. Cloning, expression and activity detection of cysteine protease inhibitor gene MdCPI in Musca domestica [J]. , 2010, 53(6): 611-617.

Characterization of the antifungal peptide MAF-1 from the hemolymph of Musca domestica larvae and its antifungal activity

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0