Abstract: The sophisticated olfactory system of insects plays a crucial role in their behaviors, such as host seeking, mating, oviposition, as well as escaping behaviors. Therefore, the mystery of arousing a series of insect behavior reactions by perceiving and distinguishing odorants in the environment will be unveiled by investigating olfactory receptive mechanism. In order to clarify the mechanism of interaction between olfactory associated proteins in Holotrichia oblita Faldermann, the HoblOBP2 bait was constructed using DUALhunter Starter Kits. Then, the normalized cDNA library from H. oblita antennae was screened. By detecting β-galactosidase activity and the Blast analysis in GenBank, six interactors were identified using HoblOBP2 bait. We inferred that the proclotting enzyme is the strongest positive interactor, which might play a role in the process of olfactory recognition.

Key words: Holotrichia oblita, odorant binding proteins, protein interaction, yeast two-hybrid system, library screening