›› 2013, Vol. 56 ›› Issue (10): 1229-1234.doi:

• SHORT COMMUNICATIONS • Previous Articles    

Virus yield parameters in mass production of three Iranian geographic isolates of Helicoverpa armigera nucleopolyhedrovirus (In English)

Ali MEHRVAR   

  1. (Department of Plant Protection, Faculty of Agriculture, University of Maragheh, Maragheh, EastAzarbaijan, Iran)
  • Online:2013-10-20 Published:2013-10-20

Abstract:  Three main parameters of virus propagation, viz., larval stage, inoculation dose and incubation temperature were studied in three nucleopolyhedrovirus isolates of Helicoverpa armigera, i. e., Maragheh (MRG), Nebrin (NBN), and Marand (MRD), collected from tomato fields of East-Azarbaijan, Iran, to select the highly promising isolate under laboratory conditions. Among the larval stages evaluated, the early 5th instar larvae recorded the maximum virus yield. The optimum inoculation dose as well as incubation temperature have been determined as 1 965.87 OB/mm2 and 25℃, respectively. However, the NBN isolate collected from Nebrin recorded the optimum mass production behavior in all the assays.

Key words: Helicoverpa armigera, nucleopolyhedrovirus, mass production, yield parameters, inoculation dose, inoculation temperature