灰飞虱唾液腺三大解毒酶家族的转录组分析

Abstract

Abstract: The small brown planthopper, Laodelphax striatellus (Fallén), is an important piercing-sucking pest of many economic Poaceae plants. Salivary glands play a key role in plant feeding for piercing-sucking pests because the secreted saliva help them pierce and digest plants and detoxify secondary metabolites. The detoxification system of insects is mainly composed of cytochrome P450 monooxygenase (P450), glutathione S-transferase (GST), and carboxylesterase (CarE). In order to clarify the expression profiles of detoxification enzyme genes in salivary glands of Laodelphax striatellus, the transcriptome of adult salivary glands was sequenced, de novo assembled and annotated, and phylogenetic analysis of the three detoxification enzyme families among L. striatellus, Acyrthosiphon pisum, and Apis mellifera was conducted in this study. The results showed that 9 glutathione S-transferases (GST) genes, 22 carboxylesterases (CarE) genes, and 39 cytochrome P450 monooxygenases (P450) genes were found expressed in salivary glands. Phylogenetic analysis showed that most of CarEs take part in dietary/detoxification and hormone/semiochemical processing while few CarEs are related to neuro/development in L. striatellus salivary glands. The number of P450 genes expressed in L. striatellus salivary glands is fewer than that in the genomes of A. pisum and A. mellifera, and only CYP6 and CYP4 genes were expressed in L. striatellus salivary glands. GSTs were the most conserved in the three insects. These results underpin the researches of adaptation to host plants and insecticides of L. striatellus.

Key words: Laodelphax striatellus, salivary gland, glutathione S-transferase, carboxylesterase, cytochrome P450 monooxygenase, phylogeny

LIU Chang-Li, LU Li-Xia, XU Yan-Li, YANG Peng-Cheng, CUI Feng. Transcriptomic analysis of three detoxification enzyme families in the salivary glands of the small brown planthopper, Laodelphax striatellus (Hemiptera: Delphacidae)[J]., 2013, 56(12): 1509-1515.

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Transcriptomic analysis of three detoxification enzyme families in the salivary glands of the small brown planthopper, Laodelphax striatellus (Hemiptera: Delphacidae)

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