Abstract: 【Aim】 In order to develop new botanical aphicides, the joint aphidicidal action of alkaloids of Sophora alopecuroides L. and nicotine against Aphis craccivora Koch was evaluated systematically indoors and outdoors. 【Methods】 Toxicities of monomeric compounds to A. craccivora were tested by topical application method. Potter spray method was adopted to test the toxicity of aphicide formulation to A. craccivora. Constant spray method was used in the field efficacy trial, which was conducted according to the national standard. Moreover, pesticide formulation processing and the measurement of the joint aphidicidal action of alkaloids of S. alopecuroides and nicotine were performed using the conventional methods. 【Results】 The mixture of alkaloids of S. alopecuroides and nicotine showed significant synergism in the aphicidal activity aqainst A. craccivora, with the common-toxicity coefficient (CTC) value of 185.82 when the optimal ratio of total alkaloids to nicotine (13∶1, w/w) was used. Based on screening on solvent and emulsifier, 15.7% total alkaloids·nicotine (TAN) emulsifiable concentrate (EC) was developed, which consisted of 14.6% alkaloids of S. alopecuroides, 1.1% nicotine, 74.3% solvent and 10% emulsifier. Quality tests confirmed that this formulation met the requirements as a commercial product. Moreover, experiments in fields showed that 0.354 g/L 15.7% TAN EC maintained 85.91% control effect on Macrosiphum rosirvorum at 7 d after application. 【Conclusion】 The mixture of alkaloids of S. alopecuroides and nicotine can significantly increase the aphidicidal efficacy each other and has the potential to be further developed.

Key words: Aphis craccivora, botanical aphicides, Sophora alopecuroides alkaloids, nicotine, aphicidal activity, Macrosiphum rosirvorum