›› 2015, Vol. 58 ›› Issue (11): 1245-1252.doi:

• RESEARCH PAPERS • Previous Articles     Next Articles

Screening and evaluation of the artificial diets of Bradysia odoriphaga Yang et Zhang (Diptera: Sciaridae)

ZHOU Xian-Hong, ZHANG Si-Cong, ZHUANG Qian-Ying, ZHANG An-Sheng, LI Li-Li, MEN Xing-Yuan, ZHAI Yi-Fan, YU Yi*   

  1. (Key Laboratory for Plant Virology of Shandong, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, China)
  • Online:2015-11-20 Published:2015-11-20

Abstract: 【Aim】 In order to solve the test sample shortage of Bradysia odoriphaga Yang et Zhang in the laboratory, we researched the artificial massive rearing technology. 【Methods】 By the controlled variable method, we screened the formulae of artificial diet. By the two-sex life table method, we compared the rearing effect between the artificial diet and the natural diet, Chinese chives (Allium tuberosum), under the conditions of temperature 25℃, a photoperiod of 16L∶8D and the relative humidity of 75%. 【Results】 We screened eight formulae and obtained the optimum ingredients and their proportions of artificial diets to B. odoriphaga: mushroom powder 6 g, Chinese chive powder 5 g, agar powder 1.25 g, sorbic acid 0.04 g, benzoate 0.05 g, yeast powder 0.5 g, vitamin C 2.5 g, and water 50 mL. This formula had similar rearing effects to Chinese chive (P>0.05). Furthermore, we compared the feeding effects of the artificial diet with the natural diet (Chinese chive) in the laboratory. The results showed that the mean generation time (T) of B. odoriphaga fed on the artificial diet was 23.6800 d, while that fed on Chinese chive was 25.9000 d, with significant difference between both (P<0.05). The intrinsic rates of increase (rm) of B. odoriphaga fed on Chinese chive and the artificial diet were 16.3200 and 41.1800/d, and the net reproduction rates (R0) of B. odoriphaga fed on Chinese chive and the artificial diet were 0.1078 and 0.1570/d, respectively. 【Conclusion】 Both artificial diet and Chinese chive can be used to rear B. odoriphaga, and the artificial diet is slightly better than Chinese chive. Moreover, the artificial diet is more economical and with low pesticide residue.

Key words: Bradysia odoriphaga, artificial diet, two-sex life table, net reproduction rate, intrinsic rate of increase, finite rate of increase