›› 2015, Vol. 58 ›› Issue (11): 1262-1272.doi:
• RESEARCH PAPERS • Previous Articles Next Articles
TANG Xiu-Juan 1, 2, JIANG Li-Yun 1, CHEN Jing 1, QIAO Ge-Xia 1,*
Online:
Published:
Abstract: 【Aim】 The aphid species in Pterocommatinae are important forestry pests. However, the aphids are in small size, their morphological characteristics tend to be simplified, and effective features for species identification are very limited, so the fast and accurate identification is hard based on external morphological characteristics. DNA barcode is an effective method for species identification. This study aims to compare the efficiency of rapid identification of Pterocommatinae based on three different molecular markers, to get the standard sequences of DNA barcode of this subfamily aphids and to solve the taxonomical problem of some species. 【Methods】 Based on COI, Cytb and Buchnera gnd genes, 197 samples of ten species in two genera were selected for the neighborjoining tree analysis, distance analysis and similarity-based DNA identification analysis under K-2P model and p-distance model. 【Results】 Compared with the K-2P model, p-distance model got the smaller genetic distance and the overlap region of intra- and interspecific divergences. COI sequences had the highest success rate of species identification. The nearly 200 standard sequences of DNA barcode for aphids of Pterocommatinae had been gotten, and the DNA barcode sequence library of the subfamily species had been built based on the three markers. 【Conclusion】 The p-distance model is superior to K-2P model, and COI sequences have the highest analysis efficiency. Plocamaphis assetacea may be a synonym of Plocamaphis flocculosa.
Key words: Hemiptera, Aphididae, Pterocommatinae, DNA barcoding; COI, Cytb, Buchnera, gluconate-6-phosphate dehydrogenase (gnd)
TANG Xiu-Juan , JIANG Li-Yun , CHEN Jing , QIAO Ge-Xia . DNA barcoding of Pterocommatinae (Hemiptera: Aphidoidea: Aphididae) based on three molecular markers[J]., 2015, 58(11): 1262-1272.
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http://www.insect.org.cn/EN/Y2015/V58/I11/1262
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