›› 2015, Vol. 58 ›› Issue (4): 454-459.

• SHORT COMMUNICATIONS • Previous Articles     Next Articles

Detection and identification of  Wolbachia in Bradysia odoriphaga  (Diptera: Sciaridae) populations from Shandong Province, China

TAO Yun-Li1, GUO Ya-Nan1, WANG Jing2, LI Li-Li2, YU Yi2,*, CHU Dong1,*   

  1. (1. Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao,Shandong 266109, China; 2. Key Laboratory for Plant Virology of Shandong, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, China)
  • Online:2015-04-20 Published:2015-04-20

Abstract: 【Aim】 This study aims to determine the infection rate and taxonomic identity of the endosymbiont Wolbachia in the populations of Bradysia odoriphaga Yang et Zhang in Shandong Province, so as to reveal the potential effects of this endosymbiont on B. odoriphaga. 【Methods】 The species identity of 12 chive maggot populations collected in Shandong was first identified based on the mitochondrial cytochrome oxidase I (mtCOI) gene. Two pairs of primers (16S-F/16S-R and 81F/691R) were used to detect the infection of Wolbachia in the 11 populations identified as B. odoriphaga. The Wolbachia infecting these B. odoriphaga populations were further identified based on the 16S rDNA sequence. 【Results】 Compared with the infection rates of Wolbachia (ranging from 6.67% to 93.33%) based on 16S-F/16S-R primers, the infection rate of Wolbachia based on 81F/691R primers was relatively low (0.00%-40.00%). Phylogenetic tree based on the 16S rDNA sequence showed that all of the Wolbachia detected belonged to group A. 【Conclusion】 This study reveals the infection rate of Wolbachia in B. odoriphaga in Shandong and their taxonomic position, and these results provide the basis for future study on the effects of Wolbachia on the biology and ecology of B. odoriphaga.

Key words: Bradysia odoriphaga Yang et Zhang, molecular identification, Wolbachia, 16S rDNA, wsp, Shandong