Acta Entomologica Sinica ›› 2019, Vol. 62 ›› Issue (2): 150-159.doi: 10.16380/j.kcxb.2019.02.002

• RESEARCH PAPERS • Previous Articles     Next Articles

Prokaryotic expression and ligand binding characteristics of odorant binding protein MmedOBP18 of the parasitoid wasp Microplitis mediator (Hymenoptera: Braconidae)

SONG Xuan1,2, SHAN Shuang2,3, WANG Shan-Ning4, TAO Yu-Xiao1,2, LI Rui-Jun1,*, ZHANG Yong-Jun2,*   

  1. (1. CollegeofPlantProtection, AgriculturalUniversityofHebei,Baoding,Hebei071000,China; 2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 3.CollegeofPlantProtection,ChinaAgricultural University,Beijing100193,China; 4.InstituteofPlantand Environment Protection,BeijingAcademyof Agriculture and Forestry Sciences,Beijing100097,China)

  • Online:2019-02-20 Published:2019-02-28

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Abstract: 【Aim】 To clarify the expression of the odorant binding protein MmedOBP18 inthe antenna of Microplitis mediator, and to investigate the ligand binding characteristics of the recombinant MmedOBP18. 【Methods】 The recombinant MmedOBP18 of M. mediator was expressed in the prokaryotic expression system. The localization of expression of MmedOBP18 inthe antenna of female wasps of M. mediator was evaluated using fluorescence immunohistochemistry assay. The binding characteristics of the recombinant MmedOBP18 protein to 99 candidate ligands were analyzed by fluorescence competitive binding assay. 【Results】 The recombinant MmedOBP18 protein was