Abstract: 【Aim】 This study aims to elucidate the regulation of multi-proteins behind diapause pupae of Lysiphlebus testaceipes, with focus on the screening of diapause-associated proteins related to energy metabolism and analysis of their functions, and is helpful to better understand the metabolic mechanism of diapause in L. testaceipes. 【Methods】 The protein contents in diapause and non-diapause pupae of L. testaceipes were compared by isobaric tags for relative and absolute quantification (iTRAQ), and the differentially expressed proteins (DEPs) in diapause and non-diapause pupae of L. testaceipes were analyzed and identified using bioinformatics methods based on GO and KEGG databases. 【Results】 A total of 135 DEPs in diapause and non-diapause pupae of L. testaceipes, including 38 up-regulated proteins and 97 down-regulated proteins, were found. GO and KEGG enrichment analysis showed that the proteins related to the terms of aspartate transport, L-glutamate transport, choline dehydrogenase activity and glycine betaine biosynthetic process from choline, and the pathway of oxidative phosphorylation were up-regulated. 【Conclusion】 The proteins associated with oxidative phosphorylation pathway are significantly up-regulated during diapause of L. testaceipes, indicating that energy metabolism is closely related to diapause. It is so speculated that oxidative phosphorylation process provides not only the main energy but also heat energy for the basic activities of diapause pupae.

Key words: Lysiphlebus testaceipes, diapause, energy metabolism, differentially expressed protein, protein content, iTRAQ