Abstract: 【Aim】 This study aims to reconstruct the phylogenetic relationships among the higher-level taxa of Fulgoroidea using low-coverage whole-genome sequencing data and transcriptome data, and to provide genome data for understanding the phylogenetic relationships of Fulgoroidea.【Methods】 The 2nd-generation sequencing technology was utilized to obtain the low-coverage whole-genome sequencing data of a species of Flatida sp. from the family Flatidae. In combination with the low-coverage whole-genome sequencing data and transcriptome data of 25 species from the Fulgoroidea (ingroup) and two species from the Cercopoidea (outgroup) downloaded, single-copy orthologous genes were extracted using BUSCO. Different matrices of completeness data based on nucleotide and amino acid sequence data were generated using Phykit to analyze the phylogenetic relationships of the Fulgoroidea. 【Results】 The number of single-copy orthologous genes in the Fulgoroidea ranged from 836 to 2 421 based on the low-coverage whole-genome sequencing data and transcriptome data. In the phylogenetic relationships of the Fulgoroidea, all phylogenetic results supported Cixiidae+Delphacidae as the sister group of other families of the Fulgoroidea, and Cixiidae, Delphacidae, Achilidae, Derbidae, Fulgoridae, Dictyopharidae, Acanaloniidae, Tettigometridae, Issidae, Caliscelidae, Ricaniidae and Tropiduchidae are monophyletic groups. However, the Nogodinidae are not a monophyletic group. Additionally, phylogenetic analyses conducted using the maximum likelihood method based on four distinct matrices suggested that the Flatidae are not a monophyletic group. Conversely, when constructing phylogeny using a species tree approach with each marker present in amino acid sequence data matrix faa_all, the Flatidae are supported as a monophyletic group, albeit with relatively low nodal support values. 【Conclusion】The phylogenetic relationships of the Fulgoroidea obtained in this study using low-coverage whole-genome sequencing data and transcriptome data are largely consistent with the previous studies. However, more specimens and molecular markers are needed to further clarify the sister group relationships among families regarding their phylogenetic relationships.

Key words: Fulgoroidea, whole-genome sequencing, single-copy orthologous gene, transcriptome, phylogeny