Abstract: 【Aim】The study aims to investigate the eclosion and reproductive behavior rhythms of Diaphania pyloalis, so as to provide a basis for the extraction and identification of sex pheromones, monitoring of population dynamics, gender communication mechanism and behavior regulation of this insect. 【Methods】The behavior rhythms of eclosion, courtship, mating and oviposition of D. pyloalis were recorded and analyzed by means of periodic continuous observation with single female adult or single pair of female and male adult timing under the conditions of light intensity 5 200 lx, temperature (25±1) ℃, relative humidity 85%±5% and photoperiod 14L∶10D. 【Results】 Under the conditions of light intensity 5 200 lx, temperature (25±1) ℃, relative humidity 85%±5% and photoperiod 14L∶10D, the eclosion rate of D. pyloalis was 91.30%, the eclosion period was about 6 d, and the peak of daily eclosion of female and male pupae was concentrated in the scotophase (2:00-6:00), the ratio of female to male pupae was 1.12∶1, and the male adult eclosion was about 0.5 d earlier than the female adult eclosion. The courtship behavior of female adults was concentrated within 3 d after eclosion, and the peak of courtship behavior was at 7-8 h in the scotophase. The courtship rate of the 2-day-old female adults was the highest (47.78%). The mating behavior of female and male adults began to appear at 1 h after entering the scotophase, and reached its peak at 7-8 h in the scotophase. The mating behavior was affected by the day-old age of adults, and the mating rate of the 2-day-old adults was the highest (53.33%). The number of eggs laid by the female adults within 1-3 d after mating accounted for more than 80% of the total number of eggs laid. 【Conclusion】The eclosion and reproductive behaviors of D. pyloalis adults have obvious circadian rhythms, which can provide a key time reference for the control of D. pyloalis, and also lay a foundation for the research and application of green control technology of D. pyloalis.

Key words: Diaphania pyloalis, eclosion, courtship behavior, reproductive behavior, circadian rhythm