›› 1998, Vol. 41 ›› Issue (3): 225-230.

• RESEARCH PAPERS •     Next Articles

AMPLIFICATION OF HOUSEFLY P-450 cDNA BY PCR DIRECTED BY A PAIR OF LOW G/C CONTENT PRIMERS

Kang Qiaohua Chen Nianchun Zhou Shunwu Qi Shunzhang   

  • Online:1998-08-20 Published:1998-08-20

Abstract: Based on multiplicity and genetic polymorphism of P-450 genes, total MrNA was isolated through oligo (Dt) -cellulose column from total RNAs of an insecticidesusceptible strain of the housefly, Musca dornestica vicina Macquart treated with sodium phenobarbital in the drinking water. Double-stranded Cdna were synthesized by reverse-transcription and used as template for polymerase chain reaction(PCR). The 1.5kb long anticipated DNA fragment coding for P-450 protein was amplified by PCR,using a pair of low G/C% content primers designed according to CYP6A1 Cdna gene.

Key words: housefly, cytochrome P-450, cDNA, PCR, genetic polymorphism