Abstract: Laboratory experiments employing playback of the recorded Second Male Vibrational Signal (SMVS) were designed here to clarify its underlying inhibition on the reproduction of Nilaparvata lugens, and the following results were obtained. (1) A substantial decrease of mating rate was observed at 1～12 h playback, but not at 24 h playback of SMVS. (2) In the stated reproduction inhibition, the normal active response to courtship signal from opposite sex was depressed greatly in female and slightly in male N. lugens tested. Additionally, much less frequent signal production was detected on the treated female, which inevitably became an obstacle to their meeting. (3) Though causing no obvious decrease of insemination rate in copulated N. lugens and the amount of oviposition, playback of SMVS surely gave rise to worse insemination quality, as indicated by smaller spermatophores and less well-developed eggs

Key words: Nilaparvata lugens, acoustic behavior, second male vibrational signal, reproduction, inhibition