Abstract: The double antibody sandwich technique, an enzyme-linked immunosorbent assay (ELISA)， was developed to detect the food relationships among 19 species of predators, 4 pest insects and 1 neutral insect in paddy fields in Dasha Township, Sihui County, Guangdong Province. The results indicated that 15 and 11 species of predatory arthropods preyed on the white-back planthopper, Sogatella furcifera, and the brown planthopper, Nilaparvata lugens, respectively. Ummeliata insecticeps, Pirata subpiraticus, Coleosoma octomaculatum，Clubiona japonocola，Dyschiriognatha quadrimaculata and Pardosa pseudoannulata were the major natural enemies of the two planthoppers. Seven predatory species preyed on Cnaphalocrocis medinalis larva, of which Clubiona japonocola, C. corrugata，Pardosa pseudoannulata, Paederus fuscipes and Casnoidea indica were the important ones. P. Pseudoannulata, P. tschekiangensis and P. subpiraticus were the major predators on nymph of Oxya sp. Chironomus sp. Was preyed by 13 predatory species. As the major or supplementary prey of predators, Chironomus sp. Played an important role in reestablishment and regulation the structure of arthropod community in paddy fields. The food relationship pattern of the assayed arthropods in paddy fields was constructed based on the results of the ELISA.

Key words: ELISA, predator, arthropod, food relationship, paddy field