Abstract: Species-specific primers of Trichogramma chilonis Ishii has been designed based on its rDNA-ITS2 sequence in this paper, and the specific primers of T. Dendrolimi Matsumura, T. ostriniae Pang et Chen and the genus Trichogramma were retrieved from previously published articles. All of the specific primers were then applied to identification of multiple Trichogramma and host eggs samples through PCR assays. The results showed that our methods could produce clear, unique and reproducible target DNA bands from individual adult wasp. Furthermore, the species-specific primer-based results were fully consistent with those derived from morphology-based ones. Thus, the molecular identification of the three important Trichogramma species in China, namely T. dendrolimi, T. chilonis and T. ostriniae, using the abovedescribed diagnostic primers was proved feasible.

Key words: Trichogramma dendrolimi, T. chilonis, T. Ostriniae, diagnostic primers, molecular identification, rDNA-ITS2