应用脂质体介导技术改变重组杆状病毒感染方法

Abstract

Abstract: InsectBaculovirus Expression System is one of the most effective eukaryotic expression systems, which has been widely used to produce a lot of recombinant proteins.The current inoculation method is to inject recombinant virus directly into silkworm larvae, resulting in possibly biological danger due to virus contamination to environment during the practical operation.Therefore, it is necessary to develop safe infection methods in order to control the diffusion of recombinant virus.In this experiment, we tried to introduce directly the genome DNA of baculovirus, instead of virus itself, into silkworm Bombyx mori larvae and got the same infection result.By using cationic lipofectin reagent, a new safe and effective infection technique without virus contamination was developed.

Key words: Recombinant baculoviruses, silkworm, inoculation technique, lipofectin

WU Xiao-Feng^1*, CAO Cui-Ping¹, CUI Wei-Zheng². An improved inoculation technique for inoculating recombinant baculovirus into silkworm, Bombyx mori, using lipofectin[J]., 2005, 48(1): 143-146.

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An improved inoculation technique for inoculating recombinant baculovirus into silkworm, Bombyx mori, using lipofectin

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