Abstract:

Based on three transgenic cotton cultivars“GK22”,“SK103” and “ZK310”and two non-transgenic, conventional cotton cultivars “SM3” and “SM12” as experimental materials, we analyzed the content of sugars and free amino acidsin these five cotton cultivars, and the secretion amount and the content of sugars and free amino acids of honeydew from Aphis gossypii Glover feeding on these five cotton varieties. The results showed the mean content of glucose, sucrose, maltose and all the soluble sugars decreased 61.76%， 89.05%，77.86% and 23.61%, respectively in the transgenic cotton cultivar “GK22” contrasted to the conventional cotton cultivars “SM3”, and decreased 14.15%，32.80%, 92.22%, 11.46% and 46.81%, 93.19%, 61.11%, 43.91%, respectively in the transgenic cotton cultivars “SK103”, “ZK310” contrasted to the conventional cotton cultivar “SM12”. There were also some differences between the mean content of each amino acid, fructose, rhamnose, trehalose and all the free amino acids in transgenic cottons and nontransgenic cottons, with some differences significant at 0.05 or 0.01 level. The results indicated non-organic insertion genes in the transgenic cottons had some influence on the synthesis of these sugars and free amino acids. The results also showed the mean amount of honeydew secreted by A. gossypii feeding on transgenic cotton variety “GK22” reduced by 40.54% contrasted to the non-transgenic cotton varieties “SM3”, and reduced by 22.67%, 30.0% respectively feeding on “SK103”, “ZK310”contrasted to “SM12”， but the mean amount of all amino acids of honeydew from A. gossypii feeding on transgenic cottons was larger than that from the conventional cotton cultivars. And the mean amount of soluble sugar, sucrose and every amino acid of honeydew from A. gossypii were different between transgenic and non-transgenic cotton cultivars.

Key words: Aphis gossypii, transgenic cotton, sugar, free amino acid, honeydew