Abstract: Cytochrome oxidase subunit Ⅱ gene segment of Aphis glycines was cloned and sequenced. The length of the sequence was 810 bp (GenBank accession no. DQ265743). The sequence was contrasted with other aphids registered at GenBank to identify their molecular homology. One pair of sequence-characterized primer was developed, and its amplified single band was about 270 bp. The specificity tests performed with this pair of primer showed the presence of the band only in A. glycines. Molecular identification of the natural enemies fed on A. glycines was tested with the specific primer. The results indicated that  the target DNA fragment of A. glycines was detected in guts of the predatory insects Harmonia axyridis, Propylaea japonica, Chrysopa septempunctata, and Orius similes, but not detected in the above predatory insects without feeding A. glycines.

Key words: Aphis glycines, cytochrome oxidase subunit Ⅱ gene, clone, natural enemy, predatory insects, molecular identification