Abstract: Cytochrome oxidase subunits 1(CO1) and 2 (CO2), and the entire sequence of the gene for tRNA leucine from twelve species of seven genera of the ant subfamily Myrmicinae were sequenced. The DNA sequences were analyzed, and the secondary structure of tRNA leucine also gene was studied. The molecular phylogenetic analyses of the subfamily Myrmicinae with Forelius chalybaeus, a species of the subfamily Dolichoderinae as the outgroup were performed by using four methods: the maximum likelihood (ML), maximum parsimony (MP), neighbor-joined (NJ), and unweighted pairgroup method with arithmetic means (UPGMA) based on the deoxyribonucleic acid data and the amino acid data. The taxonomic scheme for these genera and species based on molecular phylogeny of above genes consisted with that of the traditional taxonomy and the consistency for genera was better than that for species.

Key words: Subfamily Myrmicinae, mtDNA, gene, phylogeny