In order to solve the difficulty of the identification of necrophagous flies faced by forensic pathologists and technicians, the feasibility of species identification based on a 348 bp region of the mitochondrial COⅠ (cytochrome oxidase subunit Ⅰ)  gene using RFLP and analysis of DNA was evaluated. Samples were collected in Zhongshan, Guangzhou and Xi'an. The 348 bp region of the COⅠ gene was amplified and sequenced using forward and reverse primers. Cleavage sites for several restriction endonucleases were searched using the DNASTAR. PCR products were digested with restriction endonuclease DdeⅠ, DraⅠ and HinfⅠ, and then separated on native polyacrylamide gels. Evolutionary tree and evolutionary divergence analysis were performed using MEGA 3.0. The analysis indicated that the methods could be used to identify common necrophagous flies. The results provided basis for further research, especially for the setup of mtDNA database of insects of forensic importance.