The black-stripe-body (e^bsr) and ebony (e) are different mutants for the same gene (ebony) of Drosophila melanogaster. Both phenotypes have the similar shape, but the behavioral characteristics demonstrate significant differences between them. In this study, the black-stripe-body, ebony and wild-type flies were studied. Firstly, we detected the vision and locomotor activity of flies, and then the different mating-groups were designed for analyzing the mating success, mating latency and courtship pattern of flies. The results showed that the vision and locomotor activity were not significantly different between black-stripe-body and wild-type flies. There was no significant difference in mating success and mating latency for the black-stripe-body and wild-type flies, while there were significant differences (P<0.000) in mating success and mating latency for ebony and wild-type flies. Black-stripe-body flies perform locomotor activity and mating activity differently from ebony flies, and this may be due to the new way of the ebony gene mutation, which leads to abnormal levels of dopamine in Drosophila, thus forming the unique courtship pattern of black-stripe-body.