Abstract: 【Aim】 Yellow dwarf disease caused by barley yellow dwarf virus (BYDV) is one of the most important plant diseases that affects the production of wheat in China. GAV is the major strain of BYDV in China. Based on field investigation, BYDV-GAV, vector Sitobion avenae and non-vector Rhopalosiphum padi often occur together, which may cause serious losses to wheat yield. This study aims to investigate the activities of antioxidant enzymes and detoxification enzymes in both vector and non-vector aphids exposed to BYDV, so as to provide a reference for revealing the physiological and biochemical mechanisms of virus-aphid interaction. 【Methods】 The activities of antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT), and detoxification enzymes including acyl carrier protein (ACP), alkaline phosphatase (AKP) and acetyl cholinesterase (AchE), in S. avenae and R. padi adults fed on healthy wheat plants (the blank control group), wheat plants harmed by the 3rd instar nymphs of avirulent Schizaphis graminum for 72 h (the conditional control group) and wheat plants infected with BYDV-GAV (transmitted by the 3rd instar nymphs of S. graminum infected with BYDV-GAV for 72 h) (the treatment group) for 7 d were measured by biochemical method. 【Results】 The activities of POD, SOD and AKP in S. avenae adults fed on wheat plants infected with BYDV-GAV for 7 d were significantly increased as compared with those in the blank control group, but showed no significant difference from those in the conditional control group. And the activities of the above enzymes in S. avenae adults in the conditional control group were significantly higher than those in the blank control group. The activities of SOD, AKP and AchE in R. padi adults fed on wheat plants infected with BYDV-GAV for 7 d were significantly decreased, while the ACP activity was significantly increased as compared with those in the blank control group. And only the ACP activity in the treatment group was significantly increased as compared with that in the conditional control group. The activities of AKP and AchE in R. padi adults in the conditional control group were significantly lower than those in the blank control group, while the ACP activity in the conditional control group was significantly higher than that in the blank control group. 【Conclusion】 After feeding on wheat plants infected with BYDV-GAV, the increase of the activities of POD, SOD and AKP in vector aphid S. avenae adults is caused by previous aphid infestation, and has no relationship with BYDV-GAV. After feeding on wheat plants infected with BYDV-GAV, the decrease of the AKP and AchE activities in non-vector aphid R. padi adults is caused by previous aphid infestation, and the decrease of SOD activity is caused by the comprehensive effect of previous aphid infestation and BYDV-GAV, while BYDV-GAV and previous aphid infestation can increase the ACP activity.

Key words: Sitobion avenae; Rhopalosiphum padi; Barley yellow dwarf virus, vector, non-vector, antioxidant enzyme, detoxification enzymes