Abstract: 【Aim】 This study aims to clarify the effects of age and mating on the internal genital organs of male adults of Ostrinia furnacalis and to ascertain the relationship between the mating status of O. furnacalis male moths and sex pheromone trapping. 【Methods】 The characteristics of internal genital organs of male adults of O. furnacalis at different age (day-old) and in different mating statuses were studied with behavioral bioassay and genital anatomy and compared with those of male adults captured with sex pheromone lure in the field. 【Results】 In addition to the testis, vas deferens, vesiculae seminales, accessory glands, ductus ejaculatorius duplexes and ductus ejaculatorius simplex, the reproductive system of male moths of O. furnacalis also contains a pair of ponds of accessory gland. The primary segment of ductus ejaculatorius simplex can be divided into five segments (PS1-5), and the segment PS1 can be divided into five regions (PS1Ⅰ-Ⅴ). The genital contents in the genital duct can be grouped into five grades (grade 0-4) according to the compactness, with two statuses of the genital duct fully and not fully filled with contents. The grades of genital contents of different genital organs in 1-day-old male moth were different, but the age had no evident effect on the grade of genital contents of the reproductive system of male moth. Except for vas deferens, vesiculae seminales and ponds of accessory gland, the grades of genital contents of other reproductive organs in the newly mated male moths were significantly different from those in the unmated individuals. With the increase of time after mating, the grades of genital contents of male reproductive organs were gradually recovered, and the recovery rates of different genital organs were different. After 60 h, the grades of genital contents of the mated males were the same as that of the unmated individuals. However, until 228 h after mating, the genital contents of PS5 in the mated male moths still showed some different morphological characteristics like breaks from those of the unmated male moths. In the male moths attracted by sex pheromone lures, the reproductive system of 60.9% males showed the same characteristics as that of the unmated male moths. 【Conclusion】 The characteristics of the genital content of PS5 can be used to distinguish the mated and unmated male moths of O. furnacalis, and most of male moths of O. furnacalis attracted by sex pheromone are unmated. The results of this study lay a theoretical foundation for the determination of mating status of male moths of O. furnacalis and for pest moth monitoring and control by sex pheromone trapping.

Key words: Ostrinia furnacalis; male reproductive system, mating status, sex pheromone, mass trapping