Acta Entomologica Sinica ›› 2022, Vol. 65 ›› Issue (10): 1343-1353.doi: 10.16380/j.kcxb.2022.10.011

• RESEARCH PAPERS • Previous Articles     Next Articles

Sequencing of the complete mitochondrial genomes of Carbula crassiventris and Carpocoris purpureipennis and phylogenetic analysis of Pentatominae (Hemiptera: Pentatomidae)(In English)

LI Rong-Rong1, LI Min1, SUN Shan-Shan1, YAN Jiang1ZHANG Hu-Fang2,*, BAI Ming3,*   

  1. (1. Taiyuan Normal University, Jinzhong, Shanxi 030619, China; 2. Xinzhou Teachers University, Xinzhou, Shanxi 034000, China; 3. Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China)
  • Online:2022-10-20 Published:2022-11-27

Abstract: 【Aim】 In this study, the complete mitochondrial genomes (mtgenomes) of Carbula crassiventris and Carpocoris purpureipennis were sequenced to investigate the mitogenomic characteristics and reconstruct the phylogenetic relationships of Pentatominae. 【Methods】 The complete mtgenomes of Carbula crassiventris and Carpocoris purpureipennis were sequenced using the Illumina MiSeq Platform, and the resulted sequences were assembled and annotated. The phylogenetic trees of Pentatominae were reconstructed using Bayesian inference (BI) and maximum likelihood (ML) methods based on the sequences of the 1st and 2nd positions of the codons of the 13 proteincoding genes (PCGs) and the nucleotide sequences of the two rRNA genes of the mtgenome of the two species and other 30 Pentatominae taxa. 【Results】 The mtgenome of Carbula crassiventris and Carpocoris purpureipennis are 15 824 and 16 575 bp in length, respectively, with 13 PCGs, 2 rRNA genes, 22 tRNA genes, and a control region. The mtgenome gene arrangements are conserved within Pentatominae and no rearrangement was detected. In addition, the base composition, codon usage and RNA structures are conserved within Pentatominae. The length, types and copies of repeat units in control regions are different among species. The phylogenetic tree based on BI and ML showed a stable clade comprised of Eysarcorini, Carpocorini, Nezarini and Antestiini. 【Conclusion】 The phylogenetic analysis confirmed that Carbula belongs to Eysarcorini, and Carpocoris, Dolycoris and Rubiconia belong to Carpocorini.

Key words: Pentatominae; Carbula crassiventris, Carpocoris purpureipennis, mtgenome, phylogeny