Acta Entomologica Sinica ›› 2022, Vol. 65 ›› Issue (4): 451-459.doi: 10.16380/j.kcxb.2022.04.005

• RESEARCH PAPERS • Previous Articles     Next Articles

Sequencing and analysis of the complete mitochondrial genome of Aclerda takahashii (Hemiptera: Aclerdidae)

DENG Jun1, WANG Gang1, LU Cong-Cong1, ZHANG Jiang-Tao2, HUANG Xiao-Lei1,*    

  1. (1. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2. Key Laboratory of State Forestry and Grassland Administration on Forest Ecosystem Protection and Restoration in Poyang Lake Watershed, College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China)
  • Online:2022-04-20 Published:2022-03-24

Abstract: 【Aim】 This study aims to sequence and analyze the complete mitochondrial genome (mitogenome) of Aclerda takahashii, which represents the first complete mitogenome of the family Aclerdidae of Hemiptera, and to investigate the phylogenetic relationships with other Coccoidea groups. 【Methods】 The whole mitogenome of A. takahashii was sequenced based on the Illumina sequencing technique, and analyzed with bioinformatics methods. The phylogenetic trees of hemipteran insects were constructed based on the reported complete mitochondrial genomes of 31 speciec of 15 families of Hemiptera using the maximum likelihood (ML) and Bayesian inference (BI) methods. 【Results】 The mitogenome of A. takahashii is 16 599 bp in length with high A+T content (84.51%). The truncated tRNAs are common in this mitogenome, ten tRNAs lacking of a dihydrouridine (DHU) arm or TΨC (T) arm. The DHU and T arm of tRNAser(S1) and tRNAser(S2) are both missing. The phylogenic trees showed that Aclerdidae has the closest relationship with Coccidae. 【Conclusion】 This study reported the first mitogenome of Aclerdidae, and revealed that truncated tRNAs are prevalent in the mitogenome of A. takahashii, providing data for the further systematic research of mitogenomes of scale insects.

Key words:  Hemiptera, Aclerdidae; Aclerda takahashii; mitochondrial genome, truncated tRNAs, phylogeny