我国棉花主产区棉蚜对吡虫啉的抗性监测及抗性机理

doi:10.16380/j.kcxb.2016.11.013

摘要/Abstract

摘要： 【目的】由于生长周期短、繁殖率高，棉蚜Aphis gossypii容易对杀虫剂产生抗药性。本研究旨在明确我国棉花主产区棉蚜对吡虫啉的抗性水平及抗性机理。【方法】采用浸叶法测定了北京海淀，河北廊坊和邯郸,山东德州，河南许昌，以及新疆奎屯和阿克苏地区棉蚜对吡虫啉的抗性水平；测定了不同种群棉蚜3种解毒酶（多功能氧化酶、羧酸酯酶、谷胱甘肽S-转移酶）及乙酰胆碱酯酶的活性；并对靶标基因烟碱型乙酰胆碱受体（nAChR）β1亚基基因进行了突变检测。【结果】北京海淀、河南许昌和河北邯郸的棉蚜对吡虫啉敏感；河北廊坊、新疆阿克苏、山东德州及新疆奎屯地区的棉蚜对吡虫啉的抗性倍数(resistance ratio, RR)分别为22.6, 26.3，53.5和61.1倍，为中等水平抗性。酶活力对比研究发现，阿克苏和奎屯地区的棉蚜多功能氧化酶的比活力分别是敏感种群(北京种群)的1.7和1.8倍，羧酸酯酶的比活力分别是敏感种群的1.6和1.7倍，谷胱甘肽S-转移酶的比活力均是敏感种群的1.5倍，但是乙酰胆碱酯酶比活力在棉蚜种群间差异不显著。靶标基因突变检测表明，河北廊坊、新疆阿克苏、山东德州及新疆奎屯棉蚜种群nAChR β1亚基均存在与吡虫啉抗性相关的精氨酸到苏氨酸（R81T）突变。【结论】结果提示，多功能氧化酶、羧酸酯酶和谷胱甘肽S-转移酶活力升高以及nAChR β1亚基R81T突变与棉蚜对吡虫啉的抗性形成相关。

关键词: 棉蚜, 吡虫啉, 抗性, 酶活力, 烟碱型乙酰胆碱受体β1亚基

Abstract: 【Aim】 Aphis gossypii is easy to produce resistance to insecticides because of its short growth cycle and high reproduction rate. This study aims to clarify the levels and mechanisms of imidacloprid resistance in A. gossypii in the main cotton production areas of China. 【Methods】 Different populations of A. gossypii were collected from fields in Haidian of Beijing (BJ), Langfang (LF) and Handan (HD) of Hebei, Dezhou (DZ) of Shandong, Xuchang (XC) of Henan, and Kuitun (KT) and Akesu (AKS) of Xinjiang. Leaf-dipping method was used to determine the resistance levels of A. gossypii to imidacloprid. The activities of three detoxification enzymes ［mixed function oxidase (MFO), carboxylesterase (CarE) and glutathione S-transferase (GST)］ and acetylcholinesterase (AchE) in different A. gossypii populations were assayed. And the mutation of target gene (nAChR β1 subunit gene) was also detected. 【Results】 The BJ, XC and HD populations of A. gossypii were still susceptible to imidacloprid, while the LF, AKS, DZ and KT populations exhibited moderate levels of resistance to imidacloprid, with the resistance ratios of 22.6-, 26.3-, 53.5- and 61.1-fold, respectively. The activities of MFO, CarE and GST in the AKS and KT populations were significantly higher than those in the BJ susceptible population. The activities of MFO, CarE and GST in the AKS population were 1.7-, 1.6- and 1.5-fold, and those in the KT population were 1.8-, 1.7- and 1.5-fold as high as those in the BJ population, respectively. But there was no significant difference in acetylcholinesterase (AchE) activities among the AKS, KT and BJ populations. A single point mutation in the nAChR β1 subunit causing an arginine to threonine substitution (R81T) observed in LF, AKS, DZ and KT populations was associated with the imidacloprid resistance in A. gossypii. 【Conclusion】 The results suggest that enhancement of MFO, CarE and GST activities and the mutation (R81T) of nAChR β1 subunit might confer resistance to imidacloprid in A. gossypii.

Key words: Aphis gossypii, imidacloprid, resistance, resistance monitoring, enzyme activity, nAChR β1 subunit

崔丽, 张靖, 齐浩亮, 王芹芹, 陆宴辉, 芮昌辉. 我国棉花主产区棉蚜对吡虫啉的抗性监测及抗性机理[J]. 昆虫学报, 2016, 59(11): 1246-1253.

CUI Li, ZHANG Jing, QI Hao-Liang, WANG Qin-Qin, LU Yan-Hui, RUI Chang-Hui. Monitoring and mechanisms of imidacloprid resistance in Aphis gossypii (Hemiptera: Aphidiidae) in the main cotton production areas of China[J]. Acta Entomologica Sinica, 2016, 59(11): 1246-1253.

[1]	吴小保, 邓倩倩, 宋佳, 王骏, 叶茂. 不同浓度氯化钙浸种处理对水稻防御酶活性和抗褐飞虱的影响[J]. 昆虫学报, 2022, 65(1): 84-93.
[2]	候梦赏, 邱园妹, 赵必安, 蔚添添, 梁立强, 苏松坤, 李志国. 亚致死剂量吡虫啉胁迫对意大利蜜蜂工蜂嗅觉学习行为的影响及其脑部转录组分析[J]. 昆虫学报, 2021, 64(7): 817-827.
[3]	曹婷, 原梦颖, 杨坤, 郭磊, 褚栋. 内共生菌Cardinium对烟粉虱MED隐种耐药性的影响[J]. 昆虫学报, 2021, 64(10): 1168-1175.
[4]	朱江, 邱星辉. 昆虫抗药性相关细胞色素P450基因的表达调控机制[J]. 昆虫学报, 2021, 64(1): 109-120.
[5]	李可琢, 冯淑军, 赵艳丽, 段立清. 褐带蚜小蜂成虫生物学特性[J]. 昆虫学报, 2021, 64( 2): 240-249.
[6]	韩宝珠, 车林茸, 陈晓洁, 闫振天, 乔梁, 陈斌. 细胞色素P450基因AsCYP6Z2在中华按蚊溴氰菊酯抗性维持中的作用[J]. 昆虫学报, 2020, 63(8): 961-972.
[7]	杨付来, 赵真真, 王月华, 张兰, 张燕宁, 毛连纲, 蒋红云. 甜菜夜蛾谷氧还蛋白SeGrx原核表达、纯化及酶学性质分析[J]. 昆虫学报, 2020, 63(8): 952-960.
[8]	李妍, 龚丽凤, 王欢欢, 李曦, 孙歌, 谷少华, 梁沛, 高希武. 我国草地贪夜蛾田间种群有机磷和氨基甲酸酯类杀虫剂靶标基因ace-1的基因型和突变频率[J]. 昆虫学报, 2020, 63(5): 574-581.
[9]	高晶, 杨琼玉, 冯耀恒, 毛润乾. nC22矿物油及其与吡虫啉混用对柑橘木虱的室内毒力评价[J]. 昆虫学报, 2020, 63(2): 191-198.
[10]	何发林, 乔治华, 姚向峰, 于灏泳, 孙石昂, 李向东, 张吉旺, 姜兴印. 低致死剂量氯虫苯甲酰胺对沟金针虫食物利用和相关生理生化指标的剂量和时间效应[J]. 昆虫学报, 2019, 62(8): 948-959.
[11]	陈新华, 万炜, 冯淑军, 许胜利, 杨建军, 段立清. 四种植物精油成分对枸杞棉蚜的生物活性[J]. 昆虫学报, 2019, 62(4): 453-460.
[12]	纠敏, 汪伦记, 任利娜, 代威, 李晶晶, 赵君峰, 张敏. 烟粉虱MEAM1隐种磷脂氢谷胱甘肽过氧化物酶基因克隆与表达分析[J]. 昆虫学报, 2019, 62(2): 141-149.
[13]	李绍建, 高蒙, 王娜, 崔小伟, 桑素玲, 范腕腕, 王振宇. 河南不同地区棉蚜种群共生菌Wolbachia的检测及系统发育分析 [J]. 昆虫学报, 2019, 62(2): 215-221.
[14]	候梦赏, 蔚添添, 邱园妹, 李运贤, 李志国, 苏松坤. 亚致死剂量吡虫啉胁迫对意大利蜜蜂内勤蜂与外勤蜂免疫解毒相关基因表达及免疫解毒酶系活力的影响[J]. 昆虫学报, 2019, 62(12): 1409-1416.
[15]	梁金金, 何超, 刘少楠, 谢文, 张友军. 烟粉虱MED隐种抑制蛋白基因Btarrestin的克隆及特性分析[J]. 昆虫学报, 2019, 62(10): 1129-1139.

我国棉花主产区棉蚜对吡虫啉的抗性监测及抗性机理

Monitoring and mechanisms of imidacloprid resistance in Aphis gossypii (Hemiptera: Aphidiidae) in the main cotton production areas of China

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10