关键词:  转基因作物, 大豆, 除草剂, ZUTS-33, 节肢动物, 生物多样性； 生物安全

Abstract: 【Aim】 With the increasing of planting areas of transgenic crops, more and more attention has been paid to the ecological security issues. The study of the impact of the transgenic crops on biodiversity is not only an important component for the environmental release of transgenic crops, but also an important tool to objectively evaluate their risks. This study aims to analyze the impacts of herbicideresistant soybean on the diversity of arthropods and weeds in soybean fields. 【Methods】 From June to September 2019, we investigated the number of arthropods (the number of insects per 100 plants) and their diversity indexes (Margalef index, Shannon-Wiener index, Simpson index, dominance concentration index and Pielou index), the species and quantity of pests and natural enemies, and the density, species, quantity and diversity indexes of weeds in soybean fields planted with soybean cultivars of four treatments, i.e., the herbicide-resistant transgenic g10-epsps soybean ZUTS-33 developed in China, the receptor soybean Huachun 3 (HC-3) and the main soybean cultivar Zhonghuang 13 (ZH-13) sprayed with water, and the transgenic soybean ZUTS-33 sprayed with the glyphosate herbicide Roundup (3 000 mL/hm²), respectively, in Hefei, Anhui Province, eastern China. 【Results】 In the early and middle growth stages of soybean in 2019, the numbers of insects per 100 plants in the four treatments exhibited similar changing trends except that the number of insects per 100 plants in the treatment of ZUTS-33 sprayed with water on August 13 was significantly lower than those in the other three treatments. The number of arthropods and their various diversity indexes showed no significant difference among the four treatments at the early and middle growth stages of soybean, and the numbers of six pest groups including whiteflies, aphids, thrips, leaf beetles, leafhoppers and Helicoverpa armigera and the numbers of six natural enemy groups including spiders, ladybirds, lacewing, flower bugs, plant bugs and Anomis flava showed similar dynamics among the four treatments during the investigation period. There were 10 weed species belonging to 8 families in the investigated soybean fields. The density and number of weeds in the treatment of ZUTS-33 sprayed with Roundup were significantly lower than those in the other three treatments, and their Margalef index, Shannon-Wiener index, Simpson index, and Pielou index in the treatment of ZUTS-33 sprayed with Roundup were significantly different from those in the other three treatments at the late growth stage of soybean. 【Conclusion】 The planting of the herbicide-resistant transgenic g10-epsps soybean ZUTS-33 has no significant effect on the diversity of arthropods and weeds in soybean fields.

Key words: Transgenic crop, soybean, herbicide, ZUTS-33, arthropod, biodiversity; biosafety