番茄潜叶蛾四唑虫酰胺抗性种群的适合度及解毒酶活性

doi:10.16380/j.kcxb.2024.12.008

昆虫学报 ›› 2024, Vol. 67 ›› Issue (12): 1652-1660.doi: 10.16380/j.kcxb.2024.12.008

番茄潜叶蛾四唑虫酰胺抗性种群的适合度及解毒酶活性

朱雅婷¹, 张雅蒙¹, 李娅¹, 郭晓君¹, 张润祥¹, 吴青君², 封云涛^1,*

(1. 山西农业大学植物保护学院, 农业有害生物综合治理山西省重点实验室, 太原 030031; 2. 中国农业科学院蔬菜花卉研究所, 蔬菜生物育种全国重点实验室, 北京 100081)

出版日期:2024-12-20 发布日期:2025-01-22

Fitness and detoxification enzyme activities of the tetraniliprole-resistant population of Tuta absoluta (Lepidoptera: Gelechiidae)

ZHU Ya-Ting¹, ZHANG Ya-Meng¹, LI Ya¹, GUO Xiao-Jun¹, ZHANG Run-Xiang¹, WU Qing-Jun², FENG Yun-Tao^1,*

(1. Shanxi Key Laboratory of Integrated Pest Management in Agriculture, College of Plant Protection, Shanxi Agricultural University, Taiyuan 030031, China; 2. State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

Online:2024-12-20 Published:2025-01-22

摘要/Abstract

摘要： 【目的】本研究旨在明确四唑虫酰胺多代汰选对番茄潜叶蛾Tuta absoluta种群适合度及解毒酶活性的影响，为田间科学合理使用该杀虫剂提供理论依据。【方法】采用喷雾法在室内用四唑虫酰胺对番茄潜叶蛾汰选至18代后，用浸叶法测定四唑虫酰胺对番茄潜叶蛾2-3龄幼虫的48 h内毒力，获得番茄潜叶蛾四唑虫酰胺抗性种群；通过构建两性生命表评估番茄潜叶蛾四唑虫酰胺抗性种群适合度，并测定2-3龄幼虫体内多功能氧化酶(mixedfunction oxidase, MFO)、羧酸酯酶(carboxylesterase, CarE)和谷胱甘肽S转移酶(glutathione S-transferase, GST)活性。【结果】经过室内18代汰选，番茄潜叶蛾获得了24.60倍四唑虫酰胺抗性。与四唑虫酰胺敏感种群相比，四唑虫酰胺抗性种群的幼虫历期、蛹历期、总产卵前期(total preoviposition period, TPOP)和平均世代周期(T)均有所延长，且1龄幼虫历期和平均世代周期(T)分别显著延长0.43和0.61 d，内禀增长率(r)、周限增长率(λ)和净增长率(R₀)均下降，相对适合度为0.90，存在生存劣势。四唑虫酰胺抗性种群2-3龄幼虫体内GST和MFO活性与四唑虫酰胺敏感种群相比均显著升高，分别是四唑虫酰胺敏感种群的1.25和1.21倍，但CarE活性与四唑虫酰胺敏感种群相比无显著变化。【结论】室内抗性汰选18代后，番茄潜叶蛾对四唑虫酰胺的抗性呈不断增长趋势，其中MFO和GST可能参与了四唑虫酰胺抗性的形成。番茄潜叶蛾对四唑虫酰胺产生抗性后存在生长发育适合度代价。

关键词: 番茄潜叶蛾, 四唑虫酰胺, 适合度, 解毒酶, 抗药性

Abstract: 【Aim】This study aims to clarify the influence of multiple generations of selection with tetraniliprole on the population fitness and detoxification enzyme activities of Tuta absoluta, so as to provide a theoretical basis for the scientific and rational use of this insecticide. 【Methods】 After T. absoluta was selected with tetraniliprole for 18 generations using spraying method in the laboratory, the toxicity of tetraniliprole to the 2nd-3rd instar larvae of T. absoluta larvae in 48 h was determined by leaf-dipping method, and a tereniliprole-resistant population of T. absoluta was obtained. The fitness of the tereniliprole-resistant population of T. absoluta was evaluated by constructing the two-sex life table, and the activities of mixed-function oxidase (MFO), carboxylesterase (CarE) and glutathione S-transferase (GST) in the 2nd-3rd instar larvae were determined. 【Results】 After 18 generations of laboratory selection, T. absoluta developed 24.60-fold resistance to tetraniliprole. The larval duration, pupal duration, total preoviposition period (TPOP) and mean generation time (T) of the tetraniliprole-resistant population were all prolonged, and the 1st instar larval duration and mean generation time (T) increased significantly by 0.43 and 0.61 d, respectively, as compared to those of the tereniliprole-susceptible population. In addition, compared to the tereniliprole-susceptible population, the tereniliprole-resistant population had decreased intrinsic rate of increase (r), net reproductive rate (λ) and finite rate of increase (R₀). The tereniliprole-resistant population had a relative fitness of 0.90, indicating a survival disadvantage. The activities of GST and MFO in the 2nd-3rd instar larvae of the tereniliprole-resistant population increased significantly, being 1.25 and 1.21 times as high as those of the tereniliprole-susceptible population, respectively, while the CarE activity in the 2nd-3rd instar larvae of the tereniliprole-resistant population showed no significant change as compared with that in the tereniliprole-susceptible population. 【Conclusion】 The resistance of T. absoluta to tetraniliprole increased after selection for 18 generations in laboratory, and MFO and GST might be involved in the development of tetraniliprole resistance. The resistance of T. absoluta to tetraniliprole has a fitness cost for growth and development.

Key words: Tuta absoluta, tetraniliprole, fitness, detoxification enzyme, pesticide resistance

朱雅婷, 张雅蒙, 李娅, 郭晓君, 张润祥, 吴青君, 封云涛. 番茄潜叶蛾四唑虫酰胺抗性种群的适合度及解毒酶活性[J]. 昆虫学报, 2024, 67(12): 1652-1660.

ZHU Ya-Ting, ZHANG Ya-Meng, LI Ya, GUO Xiao-Jun, ZHANG Run-Xiang, WU Qing-Jun, FENG Yun-Tao. Fitness and detoxification enzyme activities of the tetraniliprole-resistant population of Tuta absoluta (Lepidoptera: Gelechiidae)[J]. Acta Entomologica Sinica, 2024, 67(12): 1652-1660.

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番茄潜叶蛾四唑虫酰胺抗性种群的适合度及解毒酶活性

Fitness and detoxification enzyme activities of the tetraniliprole-resistant population of Tuta absoluta (Lepidoptera: Gelechiidae)

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